How to calculate the Standard Deviation of Biological replicates?

15 June 2020 0 7K Report

hello,

I am running a qPCR experiment with triplicates on 3 biological replicates. What is the correct formula to calculate the true SD of the biological replicates that include in itself the SD of the triplicates?

thanks

Badges
Science topic

Similar topics
Correction

More Benjamin Kimelfeld's questions See All

Does anyone knows about any protandrous fish inhabiting the Mediterranean Sea, smaller than 20 cm (adult) and that change sex relatively quickly?

Other than Sparids

27 June 2024 1,809 0 View

Very urgent. Where we can get some of these plant species (details see description box) in Nigeria?

1. Vachellia drepanolobium 2. Vachellia horrida 3. Vachellia karroo 4. Vachellia nilotica 5. Vachellia oerfota 6. Senegalia polyacantha ? [email protected] +2348061166247 Thank you.

05 June 2024 4,858 1 View

How can I change the coordinate system in Abaqus using Python?

Hello, I'm writing Python scripts for Abaqus and I'm facing a problem. I need to change the coordinate system in a .odb file before extracting data but I'm stuck. I can create my coordinate system...

30 May 2024 7,431 3 View

Can you explain the difference observed between my PFGE and WGS results for E. coli comparison?

Recently i did PFGE and WGS comparison with the same set of E coli strains. In my mind, if PFGE give two different pulsotypes, it is sure that strains are not the same. But when i did WGS, i...

12 May 2024 8,107 2 View

Is there a commercial antibody against insect resilin?

Dear community, I want to perform an analysis of the risilin localization within the legs of myriapods and would like to visualize the localization of the resilin components in different...

07 May 2024 9,160 3 View

How do I specify the argument "time" in the function "create.matrix" (R package fossil)?

I would like to use the "create.matrix" function (R package "fossil") to create a matrix of taxa, localities, time and abundance. In the function, time is specified by the arguments "time.col" (=...

28 April 2024 3,545 2 View

My plasmid DNA is 96ng. How do i calculate the volume of bsa I (20000 U) to use to digest it?

I am trying to do a golden gate cloning but the ligation-digestion seems to be failing

01 April 2024 6,416 2 View

Handling procedure of brain samples for RT-PCR / Western Blot?

Hello, What would be the best procedure right after extracting brain samples from mice, if we plan to analyze the samples later ? Would it be best to just directly flash freeze the brain...

28 February 2024 7,384 1 View

What should I study to get a good introduction to combinatorial game theory?

Right now I'm working on a senior project in combinatorial game theory - I've read Knuth's book "Surreal Numbers" and I've started reading "Winning Ways for your Mathematical Plays" by Conway,...

20 February 2024 1,254 1 View

Writing a United Nation position paper for poverty and inequality Bangladesh?

What is the Current situation with poverty and inequality in Bangladesh? What are the potential sources/causes of poverty and inequality in Bangladesh? What are the consequences poverty and...

17 February 2024 4,166 0 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,508 1 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

What is the ionic radius of Hydrogen anion? And how to know the exact ionic radii of the elements?

I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...

01 August 2024 2,614 1 View

How to change the version of the article full-text pdf file?

How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...

30 July 2024 3,229 2 View

Entropy measure and QSPR modeling in Graph Theor. How to construct the table for lengthy equation?

The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...

30 July 2024 3,126 0 View

How do I correct that wrong author's profile in my publication?

I am writing to request a correction regarding the affiliation of one of my co-authors, Donald Ho. Currently, the author's profile incorrectly lists an affiliation with The Hong Kong Polytechnic...

28 July 2024 6,824 0 View

What change would occur in physics if the three different sizes of the proton and the two sizes of the deuteron accepted as new physical constants?

For people today, the enigma of the size of the proton still remains enigmatic. However, being myself a researcher in new physical theory which makes it possible to extend the different standard...

28 July 2024 7,823 1 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View