A silly question!!! Pardon please. Usually a standard curve follows serial dilution with at least four points (especially in 5 point curve) in eight and I assume it reach to 10,000 dilution from standard one. In that plate, because of specimen type, we use different particular dilution factor. If so, how to adjust with that standard curve of the same plate for readings (calculating concentration). Regardless of standard curve dilution, sample concentration only multiplied by particular dilution factor? or Should be changed the concentration of Standard 1 to match with sample dilution factor?
Win Hlaing Than Your serial dilution method is based on ten times dilution. You can try any dilution times say a series of 2 or even 5 if ten times makes the calibration standard curve beyond the range of the quantification method.
Try using this online software
https://www.physiologyweb.com/calculators/dilution_factor_calculator_no_unit.html
Win Hlaing Than Your serial dilution method is based on ten times dilution. You can try any dilution times say a series of 2 or even 5 if ten times makes the calibration standard curve beyond the range of the quantification method.
Try using this online software
https://www.physiologyweb.com/calculators/dilution_factor_calculator_no_unit.html
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