Hi there,
As there are different lysozyme assays , the definition of the unit does vary. What assay are you using?
Anyway, you should not calculate the enzyme activity in units, but in KATALS; the katal is the unit officially adopted in 1999 by the General Conference on Weights and Measures. One katal is the amount of enzyme that converts 1 mole of substrate per second. So one katal is one mole binding cleaved per second.
Well if using the spectroscopic assay based on the decrease of OD due to lysozyme induced cell lysis then katal is useless... Anyway I've been into enzymology for years and never used katal.
Thanks for your replying Dominique Liger. I use turbidity assay.
I'm asking about the blank in equation, must adding buffer or add YPD medium in reaction instead of supernatant (enzyme)
Hi again,
The blank is dependent on the enzyme solution you actually use for the assay. Basically blank contains anything from your enzyme solution but the enzyme itself. If enzyme is prepared in a buffer then buffer is used as a blank. If enzyme solution is a culture supernatant then blank should be a culture supernantant from cells not expressing lysozyme.
Thank you so much professor Dominique Liger. I will consider your suggestion. my best regards
Dominique Liger. if enzyme solution is (YPD media), I can use the medium directly. or I can use (empty plasmid + Gs115 ) culture in YPD. which one is better to use in blank.
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