What is the best protocol for a lysozyme activity assay?

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Dr Jannathulla R.

Hope, the following links may useful.

http://www.sigmaaldrich.com/technical-documents/protocols/biology/enzymatic-assay-of-lysozyme.html

http://www.biovision.com/documentation/datasheets/K236.pdf

http://www.worthington-biochem.com/ly/assay.html

https://tools.thermofisher.com/content/sfs/manuals/mp22013.pdf

Kou Hayakawa

I would like to use RP-HPLC enzyme assay using the method of file "Lysozyme by RP-HPLC". It is important to purify by RP-HPLC lysozyme and glyco-lysozyme (at leading peak). I have recently found that glyco-chains in the eucaryotic enzyme is important for the enzyme kinetics (please see file; J Chrom B rat BIN LIP Km).

Artificial substrate may be synthesized by your hand (please see files; BAQ BIN assay, and serum LIP 1).

Artificial substrate of Pyridylamination (PA-) of Chitobiose (dimer of β-1,4-linked glucosamine) may be possible to make by Pyridylamination method, which is available from the site

http://catalog.takara-bio.co.jp/product/basic_info.php?unitid=U100002865

(Takara Bio Inc., Kusatsu, Shiga, Japan).

Alaa AbdulAziz Abed

1.. Sigma-Aldrich/ Enzymatic Assay of Lysozyme (EC 3.2.1.17)

2.. Bio-Vision/ Lysozyme Activity Assay Kit (Fluorometric)