How to calculate the observed concentration (pg/ml) in Bioplex/Miliplex software?

01 August 2019 0 8K Report

Hi all,

I am working on Cytokines measurement in human plasma samples using BioPlex Magnetic bead assay (Luminex 200 system). I use BioRad kit reagents, such as standard diluent for 4-fold dilution of standard (S1-S9), Blank control (same standard diluent), sample diluent for dilution of plasma samples and so on. Now my questions are (1) how Bioplex software calculate the observed concentration? Is there any specific formula to calculate the observed concentration (pg/ml)? (2) for quantitative assay, what is the function of FI-Background in observed concentration calculation? Thank you in advance.

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