Why does exogenously applied iron salts cause leaves to become greener under sufficient iron conditions?

Do iron salts increase photosynthesis, or do they increase the size of chloroplasts, or cytochromes when iron is sufficient? In other words, is greening-up functional or structural under these...

26 January 2021 9,584 4 View

Why do my fibroblasts under hypoxia conditions proliferate slower than the same fibroblasts under normoxic conditions?

I am using healthy control lung fibroblasts in a 96 hour experiment with two drugs (nintedanib and Pirfenidone) and vehicle controls for both. I have both hypoxia (approx 1% O2) and normoxia...

12 January 2021 6,260 1 View

Mitigating future academic stressors during pedagogical development and instructional design?

Greetings from Vancouver, Canada! My doctoral research is going to begin soon. Because the instance of higher education students in Canada, and elsewhere, has been on the rise for more than a...

11 November 2020 2,600 3 View

How do you calculate the substrate concentration of an assay in enyzme kinetics given the volume and the buffer volume at a given 6mM of the enyzme?

In an attempt to plot the michaelis- menten curve, it requires the initial rate as the y-axis and the substrate contration as the x-axis. Now how do you get the substrate concentration of the...

25 October 2020 1,702 3 View

How to crystallize curcumin from ethanolic turmeric extract?

I'm having difficulty recovering curcumin from my ethanolic extract. I tried reducing its solubility in the solution by slowly adding water, but the solid just crashed out of the solution in very...

03 September 2020 3,020 5 View

Trace metal speciation?

I am carrying out a research to assess the ecological risk associated with the speciation of trace and toxic metals in floodplain suspension. Bearing in mind that the reducible, oxidizable and...

18 August 2020 4,333 4 View

What technique can be used for imaging wet hydrogels?

I'm working on alginate-based hydrogels and I want to observe the microstructures (porosity, roughness, etc.). The hydrogels were produced by gelation in aqueous calcium chloride. SEM is out of...

30 August 2019 2,863 4 View

What is the best way to hold AFM cantilevers during piranha cleaning?

Hi, I'm trying to use piranha to clean cantilevers (silicon and gold coated SiNi) but I find that bubbles form on the cantilever chips and cause them to start to float or flip over, potentially...

24 March 2019 8,787 1 View

In my DSC Isothermal tests, Avrami coefficient is (n > 4).What is the significance to this n to the growth type of the polymer?

I am not sure if I may be doing something wrong but my R-squared for primary crystallization (Xt of 3% to 20%) is about 0.9998. I am also looking to compare my data/results. Working with PLA at...

22 January 2019 9,889 5 View

Is rice bran oil good for frying?

I am considering rice bran oil for frying to replace canola oil due to its benefits. I noticed difference in the consistency of the food, in this case the quality of caramel and its adhesion to...

29 May 2018 1,311 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Is there research limitation on the relationships between digital capabilities, market orientation, brand perception and business performance?

I am looking for supporting studies discussing literature limitations on the relationships between digital capabilities, market orientation, brand perception and business performance. Also, a...

14 February 2021 7,457 5 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

What could be an interesting topic for a bachelor's thesis in a finance/marketing/computer science field?

Hi everyone! I would like to write my bachelor's thesis on a topic that's currently relevant in the sphere of finance, marketing or computer science (or if it's possible a topic concerning all the...

08 February 2021 1,300 12 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

What could be the reason for XRD pattern starting from very high counts/intensity at 3 degree 2 theta?

I am working on XRD analysis of the clay minerals from the sea sediments. My XRD pattern shows very high counts at the start of the XRD pattern (3 degree 2 theta) which is very unusual. The counts...

31 January 2021 4,493 9 View

Is it possible to prepare clay minerals by mixing pure oxides of required elements? What would be theoretical difficulties with it?

Practically this is not at all necessary. But theoretically, would hydrous/hydrothermal synthesis be enough (like mix 1 mole alumina gel and 4 mole silica gel and gently heat) or would it require...

26 January 2021 6,403 3 View

Which pump is used to transfer low melting temperature liquid metal from one container to another?

I want to transfer GaInSn alloy from one container to another with the help of an electromagnetic pump. Can anybody suggest me which brand or which company supplies these pumps?

25 January 2021 9,481 2 View

How can you accurately induce calcium overload and measure membrane potential in cultured Tg2576 neuronal cells using whole cell patch clamp method?

Hi there, I'm currently working on a project where i need to design an experiment but as i have little to no experience with this level of experimental design and no face to face help i am really...

22 January 2021 6,728 3 View