How to calculate the degree of FITC substitution to dextran?

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Suresh K Malhotra

I am enclosing one more paper. Please check

http://www.sigmaaldrich.com/technical-documents/articles/chemistry/fluorescently-labeled-dextrane.html

Hien Nguyen

Thank you Dr. Malhotra for your link. However, I still did not find the method for calculating the degree of substitution (DS) of FITC in FITC-labelled dextran in this document. Is there any formula to calculate this DS of FITC-dextran using UV spectroscopy? There is a formula in case of FITC-labeled protein, but not for FITC-labeled polysaccharides.