Can anyone suggest me a protocol with details on how to calculate the Papp=(dQ/dt)/(Ac*C0), particularly in the calculation of the index dQ/dt?
Dear Miguel,
dQ is the concentration of your tracer or marker in mol, which is detected by spectrophotemtry, and dt a duration of your experiments, usually in seconds or minutes.
I attached the file with detailed description.
Can I use concentration in terms of microgram (ug) instead of uM for entire calculation?
Does it will have any effect on final apparent permeability value?
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