01 February 2020 0 4K Report

T½ is the value of T energy for which half of the defects are produced in Cascades with energies greater than T½ and half in Cascades with energies less than T½

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CMV and EF-1alpha promoter in same plasmid?

Hi I was wondering whether CMV and EF-1alpha promoters can be put in a single plasmid? Are there any compatibility issues between the two promoters? Thank you.

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ED50 of Meropenem in Mice against Klebsiella?

I am looking for the ED50 of meropenem in mice against Klebsiella systemic/lung infection models. Is anybody aware of it? Thank you.

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What are the material characterzations that can be done on heterostructure based- materials and devices (like HEMTs, SBDs)?

Example: TEM reveals the layered stacks with different thicknesses

11 June 2023 7,653 3 View

Is there a publication that discusses expression of different glycosylation enzymes in different CHO cell lines?

Hi I am kind of looking to see if a comparative expression level analysis of the different Glycosylation enzymes of different CHO cell lines (DG44, DXB-11 and K1)is available in any publication. I...

12 April 2023 9,492 2 View

Site directed mutagenesis not working. Can anyone help?

I am trying to replace a 20 bp region with a different sequence of same lengthin a 10kb plasmid. I designed overlapping primers. However after PCR I am only getting the template. At first I...

13 February 2023 3,663 3 View

THP-1 requires differentiation before Phagocytosis assay?

I am looking to study opsonophagocytosis using THP-1 cell line from ATCC. Does the cell line require differentiation before the phagocytosis assay?

28 October 2021 3,412 5 View

Does the European heat waves impact the Indian monsoon variability?

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23 February 2021 1,283 4 View

Has anyone successfully reactivated EBV in Farage cells?

I have been able to reactivate EBV in different EBV+ cell lines in vitro. I confirm the reactivation by measuring the expression of lytic genes such as BZLF1 at protein and RNA level by Flow...

20 October 2020 8,584 0 View

How to align large plasmids (20Kb+) in Mac Vector?

I was trying to align 3 plasmids each around 20Kb in Mac Vector. I used the "Align to ref." tab followed by adding the sequences and then aligned them. However only a portion of each sequence is...

03 February 2020 2,835 1 View

How to do overlap PCR?

Hi I am trying to join two linear 1.2 kb fragments with overlaps. I read a protocol which says at least 1/2 of the PCR mix has to be the equimolar concentrations of the fragment. Why is so much...

30 May 2019 9,445 2 View

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