I’m studying population genetic structure of fish in Thailand. by DNA sequencing using mtDNA COI. How I can calculate the appropriate number of samples from 6 populations.
24 individuals will be sufficient. If you have more, multiples of of that, so easy to carry on and using instruments.
Kindly have a look at my publication on RG you get idea 'Identification of Local- and Habitat-Dependent Selection:Scanning Functionally Important Genes in Nine-SpinedSticklebacks (Pungitius pungitius)'
What I mean was 24 are sufficient. If you have more I mentioned multiples of that as easy to carry and convenient.
If you have only 30 no problem go ahead with them 24 + 6. As centrifuge accommodate 24 (different verities are there) or even any odd number or any number also no problem at all you have to organize them. Good luck!!!