How to calculate or measure the enzyme unit of any enzyme extract?

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Prakashchandra Mervana

For enzyme activity calculation:

Units (U/ml)=

(microgram of Substrate utilized/product produced) X (total assay volume) X dilution factor)

/ divided by

(volume of enzyme used) X (volume in cuvette) X (incubation time)

Shārdesh Kumar Chaurasia

Thank you Dr. Prakash :)

Sir, yet I am a research scholar. So, no Dr please

Dr means dear :p

: )