Hi,
I would like to calculate membrane potential based on Goldman-Hodgkin-Katz Equation. The problem is KCI is replaced with CsCI in bath solution and CsOH in pipette solution. How do I calculate it? Ignore potassium ions? Thanks!
Evgeny Petrov
I guess you use CsCl to block K channels. If you know the permeabilities, in/out concentration of all ions and If Cs does not permeate at all, you may simply equate to 0
permeability to Cs in GHK. Otherwise you better measure it.
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Joy Guedia (Ngwainmbi)
I agree with the other comments. I will just measure it since you've blocked K channels.
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Evgeny Petrov
Chen,
BTW, I'd advice to do both - calculation and weasurement in current-clamp mode. If you detect a big difference, you will a have an idea about how leaky is your membrane.
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