Chen Kang

7 Questions 8 Answers 0 Followers

Questions related from Chen Kang

HgCl2 to block Aquaporin 7 in beta cells?

Has anyone tried to use HgCl2 to block AQP7 in beta cells? If so, what is the concentration? Any reference link? Thanks!

21 May 2019 2,975 0 View

How to stabilize patch clamp baseline before patching?

Can someone provide me some general suggestions on how to solve the unstable baseline issue? I have unstable baseline before putting the pipette into the chamber and no suction at this time point....

15 November 2018 1,270 5 View

Calcein imaging for cell volume measurement without calcium?

Hi, I am about to do calcein imaging to measure cell volume under iso and hypo conditions. I checked online and noticed that calcein can interact with calcium. In order to prevent any undesired...

19 September 2016 5,106 3 View

How do I know whether a series resistance compensation is enough or successful?

Hi, I used HEK293 cells as an example. When I patched a cell and got a good seal (see attachment). How do I adjust series resistance compensation in order to have a successful compensation? I use...

07 June 2016 1,125 6 View

In Cre-Loxp system, does Cre only cut homozygous floxed allele?

I've read a book describing Cre-Loxp system (see the diagram). I am wondering does Cre only cut homozygous floxed allele but not heterozygous one? Thanks for answering.

05 June 2016 4,428 2 View

How to calculate membrane potential when K+ is replaced by Cs+?

Hi, I would like to calculate membrane potential based on Goldman-Hodgkin-Katz Equation. The problem is KCI is replaced with CsCI in bath solution and CsOH in pipette solution. How do I calculate...

03 June 2016 1,325 5 View

Pipette resistance setting in patch clamp?

Hi, I have a problem in pipette resistance settings. Attachments are my current settings for Axopatch 200B. When I used model cell to test, the resistance should be 10 MOhm but I only got 2 MOhm....

31 May 2016 2,471 4 View