How to calculate lysis efficiency during microbial cell lysis by high pressure homogenizer?
Hello connection While performing potency test by using BD FACS instrument some time we few population inside the gate few outside of gate and some time I got population outside of the gate I am...
17 March 2024 3,511 4 View
effect of childhood trauma on mental health
12 September 2023 5,093 2 View
Kindly provide the details
30 August 2023 4,884 1 View
I am referring Theoretical studies on electrochemistry of p-aminophenol paper for calculating the theoretical redox potential using the described in this paper. I reproduce the DFT calculations...
18 August 2023 3,573 1 View
I encountered the calculations in the research article titled "Theoretical studies on electrochemistry of p-aminophenol ." However, I'm having difficulty comprehending the procedure used to...
13 August 2023 1,639 1 View
I have a query is that after crystallization peak point some of other noise type peaks occur in DSC scan for (Se78-xTe20Sn2Znx) x=2 and x=6 ,this peak shown the material property or due to...
05 August 2023 8,945 1 View
I am trying to optimize the geometry of a reactant complex obtained from the IRC. The geometry of the product complex is getting optimized, but in the reactant complex, the maximum force is not...
23 May 2023 8,098 3 View
Hello everyone, In my case, i ligated my insert (804 bp) into pET30a expression vector (5422bp) 3:1 ratio containing 20 ul reaction by using T4 DNA ligase and incubated at 24 degree centigrade...
16 May 2023 884 3 View
Hello everyone, I have one doubt regarding cloning ? I am using pET30a (+) vector for cloning (5422bp) and Insert size 804bp. I tried all the ligation reaction ratio lilke 1:3, 1:5, 1;7 (100ng...
26 March 2023 7,023 6 View
Some journal reviewers prefer that the rate performance of batteries be expressed in C rate rather than A/g. However, since battery capacities vary across different chemistries, wouldn't A/g be a...
20 February 2023 973 1 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View