How to calculate concentration from fluorescence intensity ?

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Vishwajit Chavda

Hello Sanskruti Swain ,

As per my knowledge, To calculate concentration from fluorescence intensity, you will need to first create a calibration curve that relates the fluorescence intensity to known concentrations of the substance you are measuring.

Here are the general steps for creating a calibration curve and using it to calculate concentration:

Prepare a series of standard solutions with known concentrations of the substance you are measuring.

Measure the fluorescence intensity of each standard solution using the same instrument and conditions that you will use for your samples.

Plot a graph of the fluorescence intensity (y-axis) versus the concentration (x-axis) of the standard solutions. This will give you a calibration curve.

Measure the fluorescence intensity of your sample using the same instrument and conditions as the standard solutions.

Use the calibration curve to determine the concentration of your sample by finding the point on the curve that corresponds to the fluorescence intensity of your sample. You can do this by drawing a line from the fluorescence intensity of your sample on the y-axis to the calibration curve and then dropping down to the x-axis to read off the corresponding concentration.

Note that the accuracy of your concentration measurement will depend on the accuracy of your standard solutions and the precision of your fluorescence measurements. It's also important to ensure that the conditions are the same for both your standard solutions and your sample to minimize errors.

I hope this clears up your doubt. Good luck with your research!

Best wishes & Regards,

Sanskruti Swain

Hello Vishwajit Chavda Sir,

Thank you for your kind answer. I will try to follow your instructions and give my feedback about it. I appreciate your detailed answer. Thank you very much!

Best Regards,

Sanskruti