Is the chitosan solution soluble in the physiological environment of PBS (pH: 6.2)?

Is the chitosan solution soluble in the physiological environment of PBS (pH: 6.2)?

27 December 2020 1,263 3 View

How the transport experts define a transportation model?

a transport model (e.g logit model or ...) is based on statistical data and field works or merely based on mathematical theories or both of them? If I want to define a model ( e.g. a new model in...

19 May 2020 1,768 3 View

How can I get the Sigma Plot software training file?

How can I get the Sigma Plot software training file?

03 May 2020 5,549 5 View

can anybody help me with an interpretation of BET analysis of bulk teeth?

I did acid etching on teeth with HCL 15 % .then analyzed with BET to fined porosity size. can anybody help me with an interpretation of BET analysis of bulk teeth? I want to know if I can...

09 January 2020 6,735 2 View

How can I make a effective urea broth medium ?

Hi, I made 40% urea broth medium(Biolife Italiana) to find out the presece of helicobacter pylori in gastric biopsy but this medium responds very slowly for positive biopsy (about 2 hours) while...

07 February 2019 6,804 7 View

Heat Transfer Solid to Fluid (CFX)?

Hi, In my problem, I have defined 2 domains; solid domain (spheres) and fluid domain (the space between them). In sub domain I have defined heat source but I have a problem with its unit! I...

05 August 2018 9,824 5 View

What are some of the current hardware and software for digitization of insects in 3D?

Hi colleagues, I am working on digitization of insects. I would like to use the latest hardware and software which can produce 3D image and maintains the natural colour of the specimen. Kindly...

08 March 2018 3,018 4 View

What is the best method, condition, and strain of E. coli that can be used during transport and storage of large size plasmids "27~35 kbp?

What is the best method, condition, and strain of E. coli that can be used during transport and storage of large size plasmids "27~35 kbp. Although, I was able successfully transport the target...

05 December 2017 5,775 3 View

How to prepare the input files for running ANSYS on HPC systems?

I am currently working on a project using ANSYS workbench but finding it very difficult to run large simulations on my laptop. I was wondering if anyone has any experience on how to prepare the...

09 May 2017 2,341 1 View

How to calculate gold shell thickness of Cu-Au core-shell nanoparticles form the amount of gold precursor added?

29 October 2016 2,119 1 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Help with culturing monolayer of human brain microvascular endothelial cells (hBMEC)?

I've been having a hard time forming an endothelial monolayer with good tight junctions. The person who trained me on the culture of endothelial cells worked mostly with mouse BMEC, and he used...

22 February 2021 8,253 1 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View