How to calculate adsorption kinetics and isotherms for dye removal?

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I got cerulean gene sequenced using universal T7 promoter and terminator primers. Why Fwd primer showed mismatches while Rev did not?

The question is based on Sanger sequencing results. Gene size is 717bp which falls within the Sanger range. I was curious as to why did one of the reactions of the same sample show odd results...

01 February 2020 9,591 6 View

Gibson cloning Transformation troubleshooting. How to make it work?

My transformation after Gibson cloning isn't working. I am using NEB-alpha cells which are good for transformation but even positive control that comes with Gibson kit didn't work on the...

01 February 2020 8,711 4 View

How to extract specific type of bacteria from the filters?

If I have HEPA filter containing different types of bacteria in it and that I wish to extract Klebsiella pneumonia out of it, how do I do that?

11 December 2019 3,667 4 View

What is the reason behind "pulsing" the laser in EI or SIMS or CI?

Mass spectrometry

10 November 2018 420 0 View

Difference between Universal Primers and Degenerate Primers?

Also provide any link to good explanation of degenerate primers. Thank you.

31 December 2015 5,076 5 View

Any easy protocol to isolate Anti-HBsAg Antibodies from Human serum by Gel Filtration Method?

Specs of Column to be used for the purification of Hepatitis B anti-bodies are required.

10 November 2015 1,052 6 View

How are molecular sieves produced from bagasse (cellulosic material)?

Can these sieves be used to adsorb moisture? I am looking for the most economical method. It would enhance the surface area and adsorption capacity.

05 June 2015 6,957 5 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

In the presence of VERY high concentration (1000 ppm) of silicon, the adsorption isotherms of arsenic are showing anomalous trends, why so?

I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...

03 August 2024 6,500 3 View

How to calculate pseudo order kinetics?

I know the rate law. But for my photo-catalytic experiments, first 90min is adsorption (dark) and next 150min is under UV light (total 240min). How to remove the adsorption part for calculation?...

21 July 2024 8,807 6 View

Waht is the effect of isoelectric point on MB dye photodegradation by ZnO nanoparticles?

Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...

19 July 2024 4,868 1 View

How to decrease hydrophobicity of Ni-foam?

As, my studies are related to water adsorption, the substrates should be hydrophilic in nature. Ni-foam being hydrophobic in nature, the results are not as expected as in literature. Hence i...

14 July 2024 8,847 4 View

Help me to identify these fungal slides?

These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..

13 July 2024 9,701 1 View

Determine pore diameter by BJH of TiO2?

Dear all, l need to help me to determine pore diameter. I got from BJH data the rp(peak area), Desorption Isotherm was 1.6 nm, but from Isotherm l got type lV with hysteresis H2 loop. Can l used...

10 July 2024 2,872 1 View

Surface adsorption with the help of iron and UV/H2O2 is innovative?

1.The topic I am going to research is "Advanced Gray Wastewater Treatment Output from the Biological Process by Iron-assisted surface adsorption and UV/H2O2". I think this method has been used a...

08 July 2024 9,464 1 View

How can magnetic Biochar remove dye pollutants, is this technology beneficial?

The harmful Dye pollution from industrial processes such as textile generated how to remove with magnetic biochar ?

07 July 2024 8,118 1 View