Specs of Column to be used for the purification of Hepatitis B anti-bodies are required.
Did you ever think about affinity chromatography? You should have purified HBsAg (e.g. vaccine preparation), link it to Sepharose 4B by cyanogen bromide [Tripatzis & Horst, Nature, 1971; 231: 266] and pack into column. Then you load the source of anti-HBs antibodies, wash well with 0.15 M NaCl-0.01 M phosphate, pH 7.6 buffer and elute your purified anti-HBs with 0.15 M glycine-HCl, pH 1.8. Antibodies should be dialysed against neutral buffer and concentrated.
Part of this procedure (reverse; to obtain purified HBsAg) was described in: 'Quantitative determination of hepatitis B antigen by means of radial immunodiffusion'
K.Madalinski, WJ Brzosko, A.Budkowska, B.Mikulska; Clin. exp. Immunol. 1973; 15: 549-556. Please tell me if this advice was helpful.
Yes, I will consult with my co-supervisor first. I'll take your suggested protocol to him and work accordingly. I'll keep you updated about it. Thank you.
Yes, this way you can obtain purified anti-HBs antibodies. The method is called affinity chromatography,
KM
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