Gibson cloning Transformation troubleshooting. How to make it work?

My transformation after Gibson cloning isn't working. I am using NEB-alpha cells which are good for transformation but even positive control that comes with Gibson kit didn't work on the...

01 February 2020 8,517 4 View

I got cerulean gene sequenced using universal T7 promoter and terminator primers. Why Fwd primer showed mismatches while Rev did not?

The question is based on Sanger sequencing results. Gene size is 717bp which falls within the Sanger range. I was curious as to why did one of the reactions of the same sample show odd results...

01 February 2020 9,500 6 View

How to extract specific type of bacteria from the filters?

If I have HEPA filter containing different types of bacteria in it and that I wish to extract Klebsiella pneumonia out of it, how do I do that?

11 December 2019 3,555 4 View

What is the reason behind "pulsing" the laser in EI or SIMS or CI?

Mass spectrometry

10 November 2018 331 0 View

How to calculate adsorption kinetics and isotherms for dye removal?

06 July 2016 8,118 2 View

Difference between Universal Primers and Degenerate Primers?

31 December 2015 4,785 5 View

How are molecular sieves produced from bagasse (cellulosic material)?

05 June 2015 6,845 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View