Yasha N. Butt

8 Questions 10 Answers 0 Followers

Questions related from Yasha N. Butt

Gibson cloning Transformation troubleshooting. How to make it work?

My transformation after Gibson cloning isn't working. I am using NEB-alpha cells which are good for transformation but even positive control that comes with Gibson kit didn't work on the...

02 February 2020 8,639 4 View

I got cerulean gene sequenced using universal T7 promoter and terminator primers. Why Fwd primer showed mismatches while Rev did not?

The question is based on Sanger sequencing results. Gene size is 717bp which falls within the Sanger range. I was curious as to why did one of the reactions of the same sample show odd results...

02 February 2020 9,562 6 View

How to extract specific type of bacteria from the filters?

If I have HEPA filter containing different types of bacteria in it and that I wish to extract Klebsiella pneumonia out of it, how do I do that?

12 December 2019 3,617 4 View

What is the reason behind "pulsing" the laser in EI or SIMS or CI?

Mass spectrometry

11 November 2018 387 0 View

How to calculate adsorption kinetics and isotherms for dye removal?

I need to put my values into the standard equations but I need help with this. Anybody who is expert at it is welcome to help me out. Thank you!

07 July 2016 8,210 2 View

Difference between Universal Primers and Degenerate Primers?

Also provide any link to good explanation of degenerate primers. Thank you.

01 January 2016 5,011 5 View

Any easy protocol to isolate Anti-HBsAg Antibodies from Human serum by Gel Filtration Method?

Specs of Column to be used for the purification of Hepatitis B anti-bodies are required.

11 November 2015 1,022 6 View

How are molecular sieves produced from bagasse (cellulosic material)?

Can these sieves be used to adsorb moisture? I am looking for the most economical method. It would enhance the surface area and adsorption capacity.

06 June 2015 6,916 5 View