How to build a phylogenetic tree with more than one gene in MEGA?

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Tehseen Kanwal

Thank you!

How I can concatenate alignment?

Gaston Amzati

You should concatenate your sequences from the two genes. You can use DnaSP software to do so. Then export the concatenated file from DnaSP in FASTA or MEGA format.

Please note that your two FASTA files to be concatenated must have the same number of sequences and in the same order.

DnaSP will generates the concatenated data file that you should use in MEGA.

DnaSP website: http://www.ub.edu/dnasp/

Ahmad Mahmoudi

Well, so what if the sequences of different genes we put into MrBayes or PAUP softwares into different partions, does it work the same as concatenated datasets?

Thanks for valuable suggestions.