How to improve results of system identification procedure?

Hello, I'm performing system identification of the lateral closed-loop dynamics of a quadrotor. My model receives a setpoint and should return position and acceleration. I've proposed a second...

07 February 2021 9,791 2 View

Orbitrap Fusion FT keep getting disconnected?

Hi, I been having a problem with the Orbitrap Fusion for quite a while and it has been getting more and more frequent, which is very frusttrating. The fusion FT would get disconnected suddenly...

22 January 2021 1,235 4 View

What is the basis set to optimize ZnO using GaussView for DFT calculation study?

Greetings, I have a bit of problem in setup the basis set of my DFT study for ZnO.. As you can see in the image attached below, I use B3LYP 6-31G (d,p) for geometrical opt. of ZnO.. But sadly, I...

13 January 2021 7,508 4 View

What is the maximum time duration allowed between photoresist coating and exposure?

I have coated the Silicon wafer with photoresist (AZ1518) but until the exposure (Laser Writer) I had to wait for more than a week (Laser Writer was not operative) - would the photoresist still be...

03 January 2021 9,640 4 View

How does titanium dioxide (TiO2) as a coating's component enhance the coating performance in terms of anticorrosion??

Greetings, Recently, I was asked by a researcher how does TiO2 enhance the coatings polymeric structure making it able to reduce corrosion reaction by preventing the penetration of aggressive...

02 January 2021 5,257 5 View

What would happen if all nitrogen atoms in DNA were replaced by Carbon atoms ?

The DNA molecule is in the form of a double helix, The atoms which compose it are carbon, nitrogen, hydrogen, and phosphorus

02 January 2021 4,402 3 View

What are the suitable amplitude of sinusoidal perturbation signal to analyzed stainless steel 316L?

Greetings researchers, I want to ask for your suggestion in regards to what is the suitable amplitude of sinusoidal perturbation signal to analyzed the conditions of bare and coated stainless...

07 December 2020 7,699 7 View

How to improve DNA extraction using TRIzol and chloroform?

02 December 2020 1,919 1 View

What is the solubility of crude extract of plants in water?

Greetings all, To my limited understanding, the crude extract of plants obtained using solvents such as ethanol and methanol are usually insoluble in water. Is this correct? If it is soluble in...

02 December 2020 1,024 6 View

Which is more important for a journal? The impact factor or the quartile ranking?

I always found confusing both concepts! Which one do you think is more important? I give an example: The journal of "Theoretical and Applied Climatology" has an impact factor of 2.88, but it's a...

01 December 2020 8,608 19 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View