Please how can I justify my regression results?

I conducted a study to investigate stock market reaction to equity issues announcement and i ended up with 64 announcements and i then used the announcement day returns as my DV and investigated...

14 January 2021 1,127 3 View

How to correctly indicate a zinc finger in a protein for molecular dynamics?

I am preparing a protein that has a Zn finger for molecular dynamics. To indicate the finger of Zn a patch is placed in which the deprotonation of the cysteines is necessary so that the Zn ion can...

05 March 2020 1,425 3 View

No title

28 February 2020 1,633 2 View

How do I calculate solar absorbance and thermal emittance of absorber coating from reflectance spectrum using standard AM 1.5 and blackbody radiation?

I have a reflectance data of my solar absorber coating from UV-Vis, I want to calculate the solar absorbance of my coating using standard reference spectrum AM 1.5 and thermal emittance of thesame...

15 November 2019 225 3 View

How can Iimprove the resolution on a GC?

When I run a O2 and N2 standard in the gas chromatograph, the peaks are overlapped, How can I improve the resolution of the chromatogram. This situation is new, last monththe calibrationwent just...

22 August 2019 4,050 6 View

On a EVO LS10 SEM, I can see images on spot 0 of the aperture, however on spot 1 and 2, I cant see any images. what could be responsible for this?

I an using the EVO LS10 SEM from Zeiss, I can see images on spot 0 of the aperture, however when the aperture is switched to spot 1 or 2, I am unable to see any image. Please what could be...

05 February 2019 3,902 1 View

Where to get yeast W303-K6001 strain?

15 September 2018 3,735 2 View

My solvent-casted PVDF film was opaque and somehow shrunk during vacuum drying. What should I do?

I'm trying to make smooth PVDF film using solvent casting dried under vacuum from 20 mL of 15% PVDF in DMF solution. The vacuum dryer temperature was at 55 deg C. After some time, this is what I...

29 January 2018 803 4 View

How can I get rid of the polysaccharides on orchids tubers to isolate DNA?

I try to isolate DNA from orchids tubers, the problem is they become kind of gelatinous because the polysaccharides.... so I want to get rid of them. Thank you!

18 September 2017 9,815 3 View

Shuold the "Reads" be included or taken into consideration for calculating the researcher's score in RG?

Any RG's member

02 March 2017 6,338 8 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

What statistical test is required for an analysis on pre-COVID & post-COVID patient improvement after attending a 10-week programme?

Hi all, Myself and my supervisor are looking at carrying out a statistical analysis to see whether there is a difference in patient outcomes for our 10-week pain management programme according to...

10 February 2021 6,622 10 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View