How to avoid initial fluorescence using Hybridization Probes in LightCycler 480 in order to have a plane fluorescence until log phase?

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

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02 March 2021 1,787 2 View

How do I increase the yield of my PCR product?

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How to quantitative analize the data of TNBS assay for gelatin?

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Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

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02 March 2021 5,785 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

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Do you know good and user-friendly programs to measure plant quantitative traits?

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Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Leonhard Möckl

Could you give a little more detail about the experimental setup and the probes you use? Thanks!

Jordi Coste-Pradas

I use Hybridization Probes with FAM and LightCycler Red 640, the setup of the qPCR is 2 min at 95º and then 40 cycles with 15 s at 95º, 30s at 58º and 30 s at 72º then there is the dissociation stage. So the problem has to be in the initial cycles.

But I don't know how to avoid this basal fluorescence.