How to assemble an Arduino MEGA guitar effects pedal?
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
How to develop investments in renewable energy sources?
08 August 2024 5,112 3 View
How to design human-centered classroom in the age of A.I.?
08 August 2024 347 5 View
What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?
31 July 2024 3,533 2 View
What is human-computer interaction (HCI)?
22 July 2024 10,056 2 View
What is framework thinking?
19 July 2024 6,497 2 View
How could physics help in the achievement of sustainable development goals?
16 July 2024 8,233 1 View
How to make a Project Management Plan (PMP)?
27 June 2024 3,893 2 View
What is a Project Management Plan (PMP)?
27 June 2024 3,103 3 View
Could religion really justify crimes?
03 June 2024 2,986 6 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Hello, I am trying to clone 1334 bp insert into 6.1 kb vector using HiFi DNA assmebly/Gibson assembly kit. I am taking 100ng of vector and 1:5 molar ratio of vector: insert and 4 hrs of...
27 June 2024 9,652 2 View
To do gibson assembly cloning can I use DH5alpha strain of E. coli. for transformation?
20 June 2024 774 1 View
I am trying to do gibson assembly cloning. I have two fragments for gibson assembly cloning (plasmid backbone and insert). After gibson assembly reaction I have transformed this in E. coli....
19 June 2024 6,404 4 View
I have to join tungsten wire to metallic plate with that adhesive and entire assembly has to be heated upto 600C in ultra high vacuum. I know about zirconia paste but it is not readily available...
18 June 2024 3,092 3 View
Dear cloning community, Is it possible to use Overhangs with large Tm difference with NEB Builder HiFi DNA assembly? 5´ 26 bp Tm 57ºC 3´ 24 bp Tm 72ºC Unfortunately, extending the 5´ doesn't...
17 June 2024 8,552 2 View
I have an assembly of parts. I want to reduce the number of modes of the individual parts by CMS.
11 June 2024 2,966 0 View
I have ran the analysis a hundred times now. Still after like 200-300 replicates my software stops analysing or just closes on its own.
26 May 2024 6,125 4 View
I have this program and I use an mpu6050 connected to an arduino uno and store the accelerometer values. my problem is that the maximum sample rate I can get is 65Hz up to and with a baud rate of...
18 May 2024 644 5 View
I have been running my phylogeny tree in MEGA X. My phylogeny tree doesn't seem to have any major differences between species in plain view, but the branches are becoming more clearly divided and...
16 May 2024 1,478 1 View
