What could be the reason where i got some extra small peaks in my sample ?

Hi all, Actually, I have done the experiment where the single crystalline substrate has used to coat a thin film. In that, I have got substrate peaks and also thin-film material peaks also. But...

16 August 2020 6,532 11 View

Site Directed Muatgenesis - Deletions not working! Please help ?

Hello I have been trying to make deletion constructs using site directed mutagenesis. The largest deletion is around 522 basepairs. My plasmid is around 11KB. I have tried inverse PCR primers,...

18 March 2019 4,591 5 View

Does Koops media supports the growth of Ammonia oxidizers alone?

Does koops media supports the growth of Ammonia oxidizing bacteria only? Or other heterotrophic microbes will also grow in the same media? Similarly Watson and Waterbury media supports only NOB...

18 March 2019 4,176 1 View

Dear All, I did Nyquist plots ac impedance measurements and I found the following plot. Can anyone help me to explain the observer trend with blank?

Dear All, I did Nyquist plots ac impedance measurements and I found the following plot. Can anyone help me to explain the observer trend with different concentration on steel with blank? Thanks...

24 February 2019 7,396 2 View

How to differentiate LDPE and LLDPE through DSC?

Identification of plastic sample from environment is difficult. Differential scanning calorimetry provides a better way for identification of PP and PET and others. How to differentiate between...

06 December 2018 5,487 6 View

How to do successively FT-IR and SEM analysis of plastic film?

I want to do successively SEM and FT-IR analysis of a plastic film. Dear researchers kindly clarify which is to be done first. In case I am intended to do FTIR first and then after 20 days...

08 May 2018 8,874 5 View

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12 February 2018 9,332 5 View

Does further heating process make any structural variation in metal ferrite sample ?

05 October 2017 5,675 3 View

How do we calculate wt% for composites ?

i want to make composite with two different oxide materials in review papers one material weight is fixed in gram another material was added to different wt% so i don't know how to calculate the...

04 October 2017 3,405 5 View

Hello everyone. Is it possible to do PCA analysis for three different genes from 10 rhizosphere samples with band intensity and SW index data alone?

I have done DGGE gel analysis for three different genes from ten rhizosphere plants. I have got the data on band intensity and SW index and I would like to do Principal Component analysis (PCA)...

09 January 2017 3,543 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View