I am generating Luciferase-expressing stable Tumor Cell Lines.
First i co-transfected the Lentiviral plasmid containing the luciferase gene with 293t cell and produce the virus. Than i transduce the tumor cell line with the susp collected from from 293t transfected cell.
How should i analyzed that my tumor cell line express the luciferase successfully? I study some papers for protocol , but their plasmid have GFP reporter gene, so its quit easy to detect. But mine plasmid don't have GFP or any other reporter gene.
thanks.
Luciferase IS your reporter gene. There are numerous kits for measuring luciferase activity in live cells. See 'https://www.tebu-bio.com/blog/2016/12/05/versatile-method-to-detect-luciferase-in-reporter-cell-lines/' to start.
Dr. Raub's answer above is correct but I can hopefully add some more specifics. Simply all you need to do is lyse your cells and then add (depending on what your luciferase is) a substrate. Then simply use a plate reader to measure the luminescence values. I'm linking a specific kit that may help you out
https://www.promega.co.uk/products/reporter-assays-and-transfection/reporter-assays/dual_luciferase-reporter-assay-system/?catNum=E1910
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