I want to analyze zeta potential of methylprednisolone acetate, but each time I tried to do so the variation occurs in the same lot of the drug.
Please suggest.
Please provide more information. It's impossible to give a meaningful answer.
Are you dispersing bulk powder into water?
What is the particle size (micronized powder)?
Are the aggregates?
Do you already have a suspension?
What excipients/dispersing aids are used?
Is it a concentrated suspension and requires dilution to measure?
If you are diluting, what are you using?
What instrument?
What are the values you are getting?
Thanks John
I have using Anton Paar DLS technology instrument. Methylprednisolone acetate inj. suspension 40mg/mL
Dilution: 1mL make up to 50 mL using 1mM NaCl solution. Preservative is benzyl alcohal. Observed zeta potential is -14 mV
but it vary with each sample.
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