Hi Everyone,
I have difficulties with my current work analyzing S-Methylmethionine(SMM).
According to several reports, the analysis of SMM can be done by HPLC with a UV detector (338nm) and OPA reagent. Meanwhile, there is another report showing SMM still can be analyzed without OPA. Later on, I tried to follow the report but I failed to obtain the SMM peak chromatogram.
FYI, the system that I used in my lab:
- HPLC (THermo vanquish)
- UV-DAD detector
- Zorbax AAA column
- 40mM NaH2PO4 (mobile phase A)
- MeOH:ACN:Water (45%:45%:10% mobile phase B)
Please let me know If you guys have any information or experience about analyzing SMM without OPA.
Thank you
1h ago
Adji Baskoro Dwi Nugroho,
I would like to know why do you need analysis without using OPA. You can use OPA as it is not the harmful and can give good peak shape and response.
Feel free to ask
Dear Dr Vijay Arjun Bagul
Since my lab have no OPA and the order is still in process, thus, I came up with the idea to find another possibility to analyze SMM without OPA.
I found several references that analyzed SMM without OPA, I followed those papers but I'm still failed.
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