Hi there,

I would like to do a tumor burden follow-up by measuring circulating cell-free DNA (ccfDNA) at different time points in a mouse experiment (by treating or not mice after tumor initiation).

Does someone have a protocol to amplify DNA as I can draw only 100-200µl of blood/week in mice.

I have a protocol to isolate DNA (QIAmp DNA blood mini kit) from plasma.

Thanks in advance!

Christophe

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