Christophe Cisarovsky

3 Questions 9 Answers 0 Followers

Questions related from Christophe Cisarovsky

How to amplify circulating cell-free DNA amplification?

Hi there, I would like to do a tumor burden follow-up by measuring circulating cell-free DNA (ccfDNA) at different time points in a mouse experiment (by treating or not mice after tumor...

02 February 2016 6,836 5 View

Does anyone have a clue for a nice TMA DAB staining?

I have the following problem: I'm doing a usual DAB staining (1°Ab, 2° Ab conjugated, HRP and Tyramide and then DAB for chromogen detection) that works well. My issue is that many of the TMA...

09 September 2015 5,761 4 View

Does anyone have a protocol for double chromogenic staining using DAB and AEC on paraffin-embedded tissue?

I have one that works well for DAB using biotinylated 2°Ab, Strep-HRP and unconjugated Tyramide. (I use DAB pellet from Sigma, diulted in Tris-HCL solution). We have as well HRP 2°Ab but nothing...

04 April 2015 8,916 10 View