How to align multiple phosphorylated peptides around their phosphosites?

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How to analyze multiple phosphorilation sites?

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Does post-translational protein modification cause devisions on observed pI verses calculated pI?

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How to detect phosphorylation on Western Blots?

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Incomplete information on performance values in MCDM methods?

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Good day esteemed scholars. Still on the question on multiple linear regression analysis?

Three soil samples were used for the study and six equations were generated from the model which comprises of three for unsoaked CBR and the other three for soaked CBR. How would I know the best...

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Mark Livingstone

Clustal seems like what you are looking for.

https://www.ebi.ac.uk/Tools/msa/clustalo/

For example if you input various peptides orthologous to human Akt S473, like this:

>Human

RPHFPQFsysAsGtA

>Mouse

>Rat

RPHFPQFsYSASGTA

>Fruit Fly

EPLFPQFSYQGDMAS

>Cow

RPHFPQFsYSASATA

You get a nice output like in the attached image:

Abhijeet Singh

I just wanted to ask what parameters did you use for the clustal alignment? As I can see from the input sequences, it aligned according to the input sequences. But how to optimize it for aligning as asked in the question, i.e. "align multiple phosphorylated peptides around their phosphosites "