I have done one sphere forming assay .I have taken images using mobile of the microscopic view!kindly let me know how to add scale bar to those images if the photo is taken by mobile...
For the identification of jellyfish, mCOI, ITS1-2, 16S, 18S, and 28S primers have been used in different published research papers. Among them, I am considering selecting the primers mCOI and...
11 June 2024 4,001 5 View
How can you identify a specific variant (specific SNPs) after we have received the sequences after sanger sequence ? that is mean how we can reveal a specific variants in the sequence ? Thanks :)
30 March 2024 10,117 1 View
Can I use MZmine for preprocessing of data and GNPS for only one sample (extract) or not? and if available in GNPS which tools should i use to be able to focus on this part when watching practical...
26 January 2024 6,083 0 View
Hi, in last period we used dbSNP database in NCBI for two variants rs2304365 and rs17315309 in ST18 gene According to the dsSNP database, about the variant rs2304365 the wild type allele is C...
23 January 2024 2,138 1 View
What the free database or websites can analysis the results of sequences ?
21 January 2024 4,139 5 View
Who worked with restriction enzyme NspI, digest sequences of Human DNA? I If anyone has experience working specifically with this enzyme, that would be very helpful.
07 January 2024 270 0 View
I want to know what is more recommended for Specific SNPs (single nucleotide polymorphism) Who has good experience, What is more recommended GWAS or candidate gene? for Specific SNPs in specific...
29 November 2023 5,337 1 View
How does the integration of artificial intelligence in architecture contribute to cultural sensitivity and inclusivity by incorporating diverse cultural and social factors into the design...
19 November 2023 5,269 3 View
What are the criteria that must be considered to select the number of images in image processing techniques to make sure the proposed method is working well, and to trust the results of that model...
21 September 2023 7,271 0 View
Who uses Tetra-Arms PCR method ? Who can help us in using the method specific in using primers(outer & Inner Primers) ??
03 September 2023 10,003 5 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
i am working on ischemic stroke. for immunoflourescence i want to use zeiss confocal microscope.before i used VS120 but the images from vs120 not so good now i want to use zeiss confocal but i...
30 July 2024 2,349 1 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View