Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

Measuring viscosity and density under high pressure?

I need the density and viscosity of a molecular fluid under relatively high pressures, I expect something around 500 MPa. This kind of measurement is way outside my field, and I can find only very...

19 October 2020 2,812 3 View

What formula to get the number of masks needed to wear to prevent 1 infection?

Hi everyone ! I have a challenging time to crack the code in the following analysis...

27 July 2020 3,915 1 View

"Internal solution magnitude limit was exceeded". How to solve this question in Ansys Workbench?

I am modelling a foundation connection to a wind barrier. The connection is 4 prestressed bolts with an anchorage plate in the bottom, it is all cealed in a clump of concrete. When I change the...

29 October 2019 4,944 2 View

Does anyone know an easy and simple method of detecting trichloroacetic acid (TCA) and/or trifluoroacetic acid (TFA) in solution?

I am investigating electrokinetic migration of these acids across a supported liquid membrane (SLM), and need to determine their presence (if any) in the aqueous acceptor phase. I am thinking...

17 October 2018 9,594 4 View

Oscillating behaviour in ITC data - any explanations?

Hi, When doing ITC in our lab we have recently observed an osculating behaviour of the raw ITC data. We have tested different systems and the behaviour is the same (however, most visible with...

06 March 2018 1,117 6 View

SaOs-2 cells and lipofectamine-2000?

20 April 2016 1,781 3 View

BiFC and ImageJ threshold?

Hi, I'm currently working on the dimerization of two solute carriers. For that I'm using bi molecular fluorescence complementation. I've transfected 293FT cells attached to poly-D-lysine coated...

21 February 2016 4,440 3 View

How can I calculate functional hill numbers?

I would like to calculate the functional diversity measures proposed by Chiu & Chao (2014). The paper gives formulas for the calculation of functional hill numbers, mean functional diversity...

08 June 2015 9,731 7 View

Are there any well-documented learning algorithms for MLPs with only positive weights?

I have a 3 layer MLP architecture with which I want to approximate models for certain logics. I know that these models can be approximated using only positive weights and this property of the...

24 February 2015 4,079 15 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View