Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

Measuring viscosity and density under high pressure?

I need the density and viscosity of a molecular fluid under relatively high pressures, I expect something around 500 MPa. This kind of measurement is way outside my field, and I can find only very...

19 October 2020 2,812 3 View

What formula to get the number of masks needed to wear to prevent 1 infection?

Hi everyone ! I have a challenging time to crack the code in the following analysis...

27 July 2020 3,915 1 View

"Internal solution magnitude limit was exceeded". How to solve this question in Ansys Workbench?

I am modelling a foundation connection to a wind barrier. The connection is 4 prestressed bolts with an anchorage plate in the bottom, it is all cealed in a clump of concrete. When I change the...

29 October 2019 4,944 2 View

Does anyone know an easy and simple method of detecting trichloroacetic acid (TCA) and/or trifluoroacetic acid (TFA) in solution?

I am investigating electrokinetic migration of these acids across a supported liquid membrane (SLM), and need to determine their presence (if any) in the aqueous acceptor phase. I am thinking...

17 October 2018 9,594 4 View

How to add response bias into a repeated measures mixed anova model?

Hi everyone! I have a 2 repeated measures that was asked at 3 moments in time (within-subject factor). We doubled the measure to control for response bias. So the measure (likert scale) probed...

02 April 2018 2,389 3 View

Oscillating behaviour in ITC data - any explanations?

Hi, When doing ITC in our lab we have recently observed an osculating behaviour of the raw ITC data. We have tested different systems and the behaviour is the same (however, most visible with...

06 March 2018 1,117 6 View

SaOs-2 cells and lipofectamine-2000?

20 April 2016 1,781 3 View

BiFC and ImageJ threshold?

Hi, I'm currently working on the dimerization of two solute carriers. For that I'm using bi molecular fluorescence complementation. I've transfected 293FT cells attached to poly-D-lysine coated...

21 February 2016 4,440 3 View

How can I calculate functional hill numbers?

I would like to calculate the functional diversity measures proposed by Chiu & Chao (2014). The paper gives formulas for the calculation of functional hill numbers, mean functional diversity...

08 June 2015 9,731 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View