What are the methods to sterilize nanoparticle solution before incorporating it into MS media for seed germination studies?
Varsha Toppo
We found that seeds infected with the fungus restored their activity with the help of nanoparticles. Therefore, there is no need to pre-treat the seeds, for example, with a permanganate solution. See our article
file:///C:/Users/user/Downloads/jnep_2013_V5_04018.pdf
Yes, sterilization of the nanoparticle solution is typically required before adding to Murashige and Skoog (MS) media for seed germination to maintain aseptic conditions. Here's a short protocol:
1. Sterilisation: Filter-sterilize the nanoparticle solution using a syringe filter (typically 0.22 µm pore size) to remove any microbial contaminants.
2. Preparation of Media: Prepare and autoclave the MS media according to standard protocols.
3. Cooling: Allow the autoclaved MS media to cool down to approximately 45-50°C.
4. Addition of Nanoparticles: Add the sterilised nanoparticle solution to the cooled MS media under aseptic conditions, usually in a laminar flow hood.
5. Mixing: Mix gently but thoroughly to ensure even distribution of the nanoparticles in the media.
Ensure that the concentration of nanoparticles and the volume added do not alter the desired pH or osmotic balance of the MS media.
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