Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

In the paper mentioned can you help me to find the finite difference of equation no 19.?

In the paper "Magnetometer-Only Attitude Determination Using Novel Two-Step Kalman Filter Approach" in equation 19 do I need to complete quaternion multiplication first and then do simple...

15 June 2020 7,522 4 View

How can I calculate excited state raman intensities in Gaussian09 ?

I am doing calculation to see excited state and ground state raman intensities. I have successfully done it for ground state using dft and after finding absorption wavelength , have optimized the...

08 November 2019 7,920 3 View

How to align reverse and forward DNA sequences in chromus software ??

Need detailed procedure to align DNA seq. before performing BLAST.

09 January 2019 7,584 3 View

For detecting single nucleotide mutation, do we use traditional PCR or qPCR?

I have got a deletion of single base pair thymine at 3744 position in BRCA1 gene, which PCR technique should I use to detect our gene mutation ?

05 March 2018 3,492 3 View

How to calculate grafting % for two polymers?

Hello, I am performing grafting of two polymers using microwave assisted method. I want to know that what is the correct method to assess and calculate grafting % among the two polymers. Is...

04 February 2018 6,874 3 View

How can you ensure that the drug is completely separated from liposomes?

After doing either sephadex column or ultrafiltration. How do you ensure that entire unentrapped drug is removed from liposomes? Will there be any extra peak in DLS?

10 January 2018 1,293 6 View

Can you suggest best cost effective CO2 incubator selling company for animal cell culture?

Hi I am looking for best cost effective CO2 incubator for animal cell culture facility. There are lot of models by various companies with different features. As such, so many options and hence, a...

20 August 2017 1,957 7 View

I want to study rearrangement of ROS 1 and RET genes in FFPE sample?

24 December 2016 4,778 1 View

What is the actual molecular weight of CD63 for exosome identification?

Hii, I always get variable result for CD63 immunoblot from exosomal protein as well as cellular protein of RAW cell line macrophages.I found some paper where it mentioned that CD63 has alternative...

29 November 2016 4,536 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View