I'm currently using an optimized protocol for which cell seeding turns to be very different from my other experiments. Due to this discrepancy in cell amount, I may have to adapt concentrations of the treatment. I would be glad to receive advice or useful links. Thanks.
Dear Amandine,
you have to a little more specific about the type of cells,treatment procedure and the assay you are looking to perform for the post treatment analyses.
You could get good answers if there is more detail.
with best regards
Charan
Hi, Amandine,
first you should determined MIC (minimal inhibiting concentration) of your treatment, ea. minimal concentartion of the compound which have a visible effect of your cell of interest under your conditions (cell densitity, stage etc). This is frist step, but remebre, this concentartion may be different in different coinditions, different cell types etc. For your real experiemnts, you should use 2-3 times higher concentration (tocompare with MIC), but you should not go over 3 times, because you will have a side effect , ea. interatctions with not-specific receptor. Good luck!
Dear Charan,
Thanks for your suggestion, actually I have to plate 2/3 less primary culture than usually.
Hi Taras,
Thanks for your advice, I should definitively determine the MIC !
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