I am looking for someone who has already used an anti-centrobin antibody to label centrioles on brain tissues?
I can only help two scientific articles on the subject (in the annex to the letter). All the best.
Hi researcher, We need you, have you already observed a different inflammatory response according to the presence or not of coating (collagen in our case) from human primary keratinocytes...
18 December 2022 3,766 1 View
Hello, I am using an ICP-OES for elemental determination for waste water samples. The procedure is to digest 100 mL of the waste water with 2 mL HNO3 + 1 mL HCl, until the volume is reduced to 20...
16 September 2021 3,236 5 View
Hello everybody, I have a single question! I have already do my experiment with single si RNA ( 3 diferents siRNA by target). first part done! Now, to go further, I have to continue with...
28 September 2020 8,552 4 View
Hi, I have been doing RNA isolation from human EDTA plasma using TRIzol LS and I am having some issues with the RNA quantification... I have measured RNA amount (by using 1µL of sample) with...
16 June 2020 2,092 3 View
Hello everyone, I am looking for a surgery protocol to implement a canule mouse caecum. Is any of you have percutaneous endoscopic caecostomy protocol in murine models? I want to do a gavage...
20 June 2019 353 0 View
I'm currently working on how plants can find carbon sources for their metabolism, except from photosynthesis. I found cases of mycoheterotrophic nutrition in non-chlorophylic plants and orchids,...
20 March 2018 1,515 4 View
Which common agents can i use to achieve cell cycle arrest (not apoptosis) in HCT116 cell line for all the cell cycle stages? Looking to do a chemotherapeutic drug response for each stage
23 September 2017 673 3 View
Hello, I performed a titration of FCCP in HEK cells and two other melanoma cells lines in order to do a mito stress test with the Seahorse technology. While I can clearly see an increase in OCR...
30 August 2017 881 10 View
I have some problem with Gibson assembly reaction. I work on viruses, and virus genome do some secondary structures and hairpin sometimes. As we have a only reaction at 50°C for Gibson assembly, I...
01 August 2017 6,185 25 View
I think it's a Gonyaulax because of it's very angular shape and it's ornamented theca, but if someone can help me to identify this cell, I will thank you. This cell has been found in a brackish...
14 March 2017 4,496 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
Information in The Three Dimensions of Time.Information in The Three Dimensions of Time Information Transfer based on Brains Entanglement.Information Transfer based on Brains Entanglement Time...
28 July 2024 7,522 0 View