Thanks for your response, the papers that you sent are useful, but I am still stuck on how to activate the DEAE-52 to do the purification or fractionate the polysaccharides? Should I activate the DEAE-52 with up-distiller water?
The DEAE-52 was activated, firstly immersed in distilled water for three hours, then inundated in 0.5M NaOH for two hours, washed until reached pH=7, inundated again for two hours in 0.5M HCl, washed until reached pH=7, finally was settled into the column.