Hello dear researchers,
I am using dark field illumination to get the reflection signal for the left hand and right hand circular polarized light using a 0.90 NA, 100x Objective. I have read that if you will make your beam tightly focused to the sample , it will no more circularly polarized light. Why is so? and the scheme which i am using to collecting the light is enough to measure the CD spectra. My structure size is about 450 nm.
Thanks