Usually I wait the agar solution to cool down bellow 50 °C to add kanamycin and pour on the petri dishes. However, I wanted to know if this is really necessary, providing kanamycin is more stable than other antibiotics, such as ampicillin.
I really do not how stable kanamycin is, I always wait to cool down the medium. However you can make a trial cooling and not cooling the medium down and test two strains, one resistant and one not. This will allow you to determinate the stability of the kanamycin and you just need little time to perform it.
Kanamycin activity is stable at ph 7.3 and 72 degrees C.
From the paper cited by Sebastián Finger :
"""
The half-life (t1/2) at pH 7.3 and 72 degrees C ranged from 3.3 h (k = 7.26 day-1, where k [degradation constant] = 1/t1/2) for ampicillin to no detectable loss of activity for kanamycin, neomycin, and other antibiotics.
"""
Article Stability of antibiotics under growth conditions for thermop...