Do you recommend to use imidazole buffer in RP HPLC?

I am working with 75% acetonitrile and 25% 10 mM citrate buffer (pH 7) and I have had pressure problems going to 90% organic. Imidazole has a pka= 7, but is not used as buffer in HPLC, i don't...

12 April 2020 932 3 View