Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

How to classify and recognize unseen image class in document sorting?

To sort the scanned document image I have to determine the class of document. But no of classes of image will keep on adding even after training. So at time of inference first I will detect unseen...

16 February 2021 4,501 3 View

Which one is more effective solvent in water and methanol for garlic extraction?

kindly explain it

03 February 2021 2,034 3 View

Why pink coloured or light red onion have more storage life than white and dark red one and smaller size one to has more than bigger one?

It is generally seen light coloured varieties has more storage life.what's relation with colour and size with storage life.

13 December 2020 634 18 View

How to solve MSTRAT software Running problem?

Hello, we have been trying to run MSTRAT Software for developing a core collection of Chilean Garlic genetic resources, but we keep getting the error: ether couldn't execute...

25 November 2020 9,748 2 View

Effect of nitrogen on onion storage?

Excessive nitrogen can reduce storage life of onions due to diseases and skinning. I am looking for research papers on tis topic.

23 January 2020 1,045 2 View

ANOVA: are groups with one sample disallowed?

I am performing a one-way ANOVA on ten groups of samples (onion yields - with onion variety as the dependant factor). The number of samples in each group varies between two and four; with some of...

10 November 2019 4,828 4 View

ANOVA: are groups with one sample disallowed?

I am performing a one-way ANOVA on ten groups of samples (onion yields - with onion variety as the dependant factor). The number of samples in each group varies between two and four; with some of...

04 November 2019 2,834 3 View

How to make different concentration of silver nanoparticle (AgNP)?

Hello, I am interesting to make a research about AgNP antibacterial activity using the green synthesis method for my thesis. I've read from several publications, they use various concentrations....

14 October 2019 1,321 8 View

I want tips to ensure a successful experience in Allium cepa assay can you help me please?

I did the Allium cepa assay but didn't see any chromosomal abnormalities or divisions, I want tips to ensure a successful experience and What is the best dye? Acetocarmine or Basic fuchsine?

09 October 2019 9,914 3 View

How to make different concentration of silver nanoparticle (AgNP)?

Hello, I am interesting to make a research about AgNP antibacterial activity using the green synthesis method for my thesis. I've read from several publications, they use various concentrations....

09 October 2019 3,664 10 View

Which solvent will dissolve my n-hexane garlic extract?

I extracted bioactive compounds from garlic by using n-hexane and evaporated it afterwards. However, the concentrated extract is not fully dissolved as I can see precipatates floating in...

22 August 2019 1,965 12 View