Stephen Samuel ANOVA after data transformation by Box-Cox

Osborne, J. (2010). Improving your data transformations: Applying the Box-Cox transformation. Practical Assessment, Research, and Evaluation, 15(1), 12. Yu, H., Sang, P., & Huan, T. (2022). Adaptive Box–Cox Transformation: A Highly Flexible Feature-Specific Data Transformation to Improve Metabolomic Data Normality for Better Statistical Analysis. Analytical Chemistry, 94(23), 8267-8276.

Stephen Samuel I would suggest Multifactor ANOVA (Two-Way ANOVA) could be a better choice. By conducting a Two-Way ANOVA on the data, you can examine whether there are significant differences in the metals concentrations among the three plants and whether there are significant variations based on different locations. Additionally, the analysis allows you to identify any potential interaction effects between the genotype and location.