we have synthesized 27Mer peptide which contains 8 glycine amino acid. and due to inefficient coupling one of the glycine coupling was partially completed giving us the peak of desglycine peptide.
The retention time in HPLC for the both is same. we have tried different methods , buffers and organic modifiers.
if anyone has encountered a same problem and solved it please let me know.
If you're using microwave assisted peptide synthesizer then I must recommend to increase the coupling reaction time after each successive glycine coupling or you can just let the sps resin aggitated under nitrogen bubbling condition with the reactant amino acid (glycine) solution for 10 to 15 minutes before applying microwave power and temperature of your actual coupling step so that you can ensure the maximum coupling extent...I have found this one quite useful during long peptide synthesis....
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