Interdendritic phase coarsens during homogenization?

Hello Is it possible for the micro-segregation (non-equilibrium phases) coarsen during the homogenization treatment? There is an obvious reduction in the quantity, but some of the phases remained...

19 September 2020 4,985 3 View

Preparing ethanol standards for HPLC?

I need to prepare ethanol standards for HPLC to quantify the bio-ethanol in my sample of interest. What range of known concentrations would I have to prepare for estimating ethanol concentration...

15 October 2019 8,844 5 View

Quantitative data from a regular FT-IR?

Hi, ATR-FT-IR spectroscopy is being used for the quantification of a particular compound. Is it not possible to run a known quantity of a sample in a regular FT-IR and then compare the peaks...

22 August 2019 9,552 17 View

Can anyone help me with an SDS PAGE's image?

Hi, One of my colleagues has used a pre-cast, commercially available 10% separating gel for his SDS PAGE. Later, while interpreting the results, we observed a broadening of the marker lane...

14 August 2019 8,943 17 View

Grid size in material diffusion simulation - Physical significance?

10 October 2018 8,213 1 View

Is it necessary to prepare all the biological replicates on the same day?

I am wondering whether it is completely necessary to have a biological replicates for an RNA seq experiment to be prepared on the same day. What if in case of a differential gene expression...

20 September 2018 1,825 5 View

Chemical mobility from Darken's equation?

19 June 2018 8,585 3 View

Why 002 reflections are absent in spinel oxides (FCC)?

For example, magnetite (Fe3O4) does not contain 002 reflections but contains 004 reflections, though it has FCC structure. Is it because of the structure factor? In my case, I have an oxide that...

29 April 2018 2,053 4 View

Can i use anastrazole steroid used by bodybuiders to study its effect in rat breast cancer model?

I have to give oral dosage of anastrazole for three months.

29 December 2017 4,977 1 View

What is the difference between graphene and carbon quantum dots??

both quantum dots have the same graphite structure with lattice spacing around 0.34 nm. how can we differentiate?

01 October 2017 3,107 7 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View