I am currently planning some lab work that requires me to dissolve tris(2,20 -bipyridyl) ruthenium(III) chloride hexahydrate dye in a solvent in order develop a calibration curve relating phosphorescence lifetime and quencher concentration (In this case O2 is the quencher). I'm currently trying to figure out what solvent is best for the application. My background is in biomedical engineering and not chemistry, so my reasonably basic knowledge chemistry is not much help!
I had considered water as a solvent but figured the presence of dissolved O2 would potentially lead to inaccuracies in the oxygen concentration for the calibration curve. If I used a Schlenk line of nitrogen to remove the dissolved O2, would degassed water be a viable option for the solvent?
In regards to mixing O2 with the solvent and maintaining a desired O2 concentration within the solution, I have read articles where a mass flow controller was used to achieve desired O2 concentration, can anyone confirm this method? Any suggestions / guidance is greatly appreciated!
You probably mean tris(2,20 -bipyridyl) ruthenium(II) chloride hexahydrate.
Do you want to use the O2-probe? What is the instrument? The calibration will depend on solvent. Water is OK. Use Ar to purge the solution.
"I have read articles where a mass flow controller was used to achieve desired O2 concentration, can anyone confirm this method?" I have published several papers using this approach.
Yes, ruthenium(II) chloride was intended. The goal is to use it for
O2 sensing in a microfluidic device. Is there any reason why you recommend Ar instead of Nitrogen for purging the solvent of is either option viable? An Olympus CKX53 fluorescence microscope will be used for the experiment.
I would not recommend to add [Ru(II)(bpy)3]2+ to your sample. Illumination of your sample with 454 nm light will result in an extremely complex photochemically initiated processes in your sample. In commercially available O2-sensors the sample is separated from [Ru(II)(bpy)3]2+ solution by a membrane. Such a sensor is a pretty complex device but not very expensive (several hundred US$).
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