I am going to isolate T cells from skin using MAC isolation kit; however, I will get the mouse skin two days before the experiment. Do not know how can I preserve them in a way that will not affect the separation and flow cytometry analysis later.
Jolien Suurmond
Will you isolate the T cells on the day you get the skin? I think that is preserved. You can try to keep your isolated T cells, or total cells from skin in normal culture medium. What kind of experiment will you be doing the 2 days later?
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Fatma Hamed
Hi Jolien,
Thx for ur answer. I wont do the isolation on the same day and that is the problem. is there any way I can store the tissue for T cells isolation that I ll 2days later?
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