Fatma Hamed

5 Questions 19 Answers 0 Followers

Questions related from Fatma Hamed

Which buffer can I use to store cells overnight after Fix-perm step?

I am doing Flow-cytomteric staining of PBMCs for extra- and intra-cellular markers. I have read that I can stop the protocol after fixing and permealising the cells (fix-perm step), but I am just...

03 March 2017 9,075 5 View

How to perform dual staining for Intra-/Extra-cellular markers (e.g FOXP3 CD3) on paraffin embedded sections?

Hi all, It would be greatly appreciated if someone can please share a protocol for double staining of Intracellular marker (FOXP3)/(extracellular marker) CD3 on paraffin embedded section for...

03 March 2017 5,770 8 View

How do the bands look like for pouring gradient gel technique in order to investigate broad range MW proteins on the same gel?

Hi, I would like to look at two different proteins with broad MW (10 and 100KDa) on the same gel. I looked on YouTube and found a video below where you can pour the high and low gradient SDS gels...

09 September 2016 6,466 3 View

Can I test protein expression in T cells by western blotting after their activation by T cell activation kit?

I am panning to maintain CD4 Cells in culture for 10 days using T cell activation/expansion kit from Miltenyi to test the effect of a treatment on some proteins by Western blotting, so I am...

04 April 2015 3,643 2 View

How should I store my tissue or cell suspension for flow cytometry/ cell separation?

I am going to isolate T cells from skin using MAC isolation kit; however, I will get the mouse skin two days before the experiment. Do not know how can I preserve them in a way that will not...

05 May 2014 8,465 2 View