The aim of my experiment is to detect the expression of genes in cancer stem cells. I will collect fresh clinical tumor specimen from the hospital and it will take about six hours before separation of the single cell and incubation with CSC marker. But the problem is, no machines are available now as all the technicians are in off duty. So I have to separate the sample on the other day. However, for this kind of situation, the amount of single cells will decrease dramatically. Can anyone suggest a good solution to store the sample overnight?
Hi. If you want to culture the cells the next day and then carry your experiment, then the sample can be stored in HBSS @ 4⁰C. If you want to do gene expression studies directly from the specimen, then put the sample in RNA Later. It will stabilize and protect RNA till you proceed with the experiment.
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